Abstract
The occurrence of the diamondback puffer Lagocephalus guentheri Miranda Ribeiro, 1915 in the Greek waters of the Aegean Sea is herein confirmed through DNA barcoding based on a 608 bp fragment of the mitochondrial COI gene of a specimen collected on 14 September 2025 along the northwest coast of Rhodes Island, southeastern Aegean Sea, Greece. Morphological traits are evaluated alongside molecular data, with all nucleotide sequences used in the phylogenetic analysis consistently supporting the identification of L. guentheri. The present study aims
to clarify the existence of this species in the region and to contribute additional reference sequences to public databases.
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